A microfluidic wound-healing assay for quantifying endothelial cell migration
نویسندگان
چکیده
منابع مشابه
A microfluidic wound-healing assay for quantifying endothelial cell migration.
Endothelial migration is an important process in the formation of blood vessels and the repair of damaged tissue. To study this process in the laboratory, versatile and reliable migration assays are essential. The purpose of this study was to investigate whether the microfluidic version of the conventional wound-healing assay is a useful research tool for vascular science. Endothelial cells wer...
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Endothelial cell (EC) plays an important role in various biological phenomena, which are highly regulated by oxygen microenvironments. For instance, tumor angiogenesis initiated by hypoxia microenvironment induces migration of ECs to form blood vessels. Previous studies suggested that hypoxia induces migration of ECs by vascular endothelial growth factor (VEGF) under different oxygen tensions. ...
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This paper presents a tubing-free microfluidic wound healing assay to quantify the migration of vascular smooth muscle cells (VSMCs), where gravity was used to generate a laminar flow within microfluidic channels, enabling cell seeding, culture, and wound generation. As the first systemic study to quantify the migration of VSMCs within microfluidic environments, the effects of channel geometrie...
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ژورنال
عنوان ژورنال: American Journal of Physiology-Heart and Circulatory Physiology
سال: 2010
ISSN: 0363-6135,1522-1539
DOI: 10.1152/ajpheart.00933.2009